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应用单克隆抗体(OKT系统)借助葡萄球菌——免疫球蛋白(SACI-Ig)花环法对同一正常人分10次采血检查外周血淋巴细胞亚群,将三种方法存放标本24小时后检查结果与新鲜血检查结果进行了比较。结果:全血4℃存放24小时后分离淋巴细胞,结果有显著改变;先分离淋巴细胞,用 NS·1640稀释,37℃ 24小时后,结果有轻度改变;先分离淋巴细胞,用单纯 1640稀释,37℃24小时,其结果无明显变化。
Application of monoclonal antibody (OKT system) by staphylococcal - immunoglobulin (SACI-Ig) garland method for the same normal 10 times the blood to check the peripheral blood lymphocyte subsets, the three methods were stored 24 hours after the test results Compared with the fresh blood test results. Results: The whole blood was stored at 4 ℃ for 24 hours and then the lymphocytes were separated. The results were significantly changed. First, lymphocytes were separated and diluted with NS · 1640. After 24 hours at 37 ° C, the results were slightly changed. Lymphocytes were separated first, Dilution, 37 ℃ 24 hours, the results no significant change.