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钙离子载体Ａ２３１８７可以引起细胞内Ｃａ２＋浓度升高，并可诱导某些细胞程序死亡。本文发现１ｇ／ｍｌＡ２３１８７作用于ＨＬ－６０细胞４小时即可诱导程序死亡，以无毒性浓度的蛋白磷酸酶２Ｂ（ＰＰ２Ｂ）的抑制剂环抱菌素Ａ（ＣｓＡ）（０．５－３ｇ／ｍ１）预处理可以抑制Ａ２３１８７诱导的ＨＬ－６０细胞程序死亡，磷酸酶１、２Ａ、２Ｃ的抑制剂ｏｋａｄａｉｃａｃｉｄ（ＯＡ）和酪氨酸磷酸酶的抑制剂钒酸钠（ｓｏｄｉｕｍｏｒｔｈｏｖａｎａｄａｔｅ，ＳｏＶ）则无此效应。流式细胞术测定群体细胞内总Ｃａ２＋变化发现，ＣｓＡ不抑制Ａ２３１８７诱导的胞内Ｃａ２＋浓度升高，表明ＣｓＡ作用于Ｃａ２＋升高后的下游事件。
Calcium ionophore A23187 can cause elevated intracellular Ca2 + concentration and induce some programmed cell death. It was found that 1g / ml A23187 could induce programmed death in HL-60 cells for 4 hours, and cyclosporin A (CsA) (0.5-3g / ml) could be induced by non-toxic inhibitor of protein phosphatase 2B (PP2B) Pretreatment could inhibit apoptosis induced by A23187 in HL-60 cells. No effect was found for okadaicacid (OA), an inhibitor of phosphatase 1,2A, 2C and sodiumorthovanadate (SoV), a tyrosine phosphatase inhibitor. The results of flow cytometry showed that CsA did not inhibit the increase of intracellular Ca2 + concentration induced by A23187, indicating that CsA acts on the downstream events of Ca2 + upregulation.