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目的:观察化瘀止痛方对人子宫腺肌病病灶细胞前列腺素F2α(PGF2α)和前列腺素E2(PGE2)含量的影响。方法:实验分为化瘀止痛方高、低浓度组,空白对照组,基质组及米非司酮西药对照(R U486)组,每组5例,采用胶原酶消化法培养人子宫腺肌病病灶细胞,检测药物干预前后子宫腺肌病病灶细胞PGF2α、PGE2的表达。结果:化瘀止痛方高、低浓度组PGE2、PGF2α含量与空白对照组比较,差异均有显著性意义(P<0.05)。化瘀止痛方高浓度组PGE2含量与RU486组比较,差异有显著性意义(P<0.05);化瘀止痛方高、低浓度组子宫腺肌病细胞上清中PGF2α含量与RU486组比较,差异均有显著性意义(P<0.05)。结论:化瘀止痛方降调子宫腺肌病细胞PGE2、PGF2α分泌,从而使子宫恢复正常收缩功能,此可能是其止痛的主要机理之一。
Objective: To observe the effect of Huayu Zhitong Recipe on the content of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) in human adenomyosis cell line. Methods: The experiment was divided into high and low concentration group, low concentration group, blank control group, matrix group and mifepristone western medicine control group (R U486), with 5 cases in each group. Human adenomyosis was induced by collagenase digestion Focal cells, detect the expression of PGF2α and PGE2 in the adenomyosis cells before and after drug intervention. Results: Compared with the blank control group, the contents of PGE2 and PGF2α in Huayu Zhitong Fang high and low concentration groups were significantly different (P <0.05). Compared with RU486 group, the content of PGE2 in high concentration group of Huayu Zhitong Fang group was significantly lower than that of RU486 group (P <0.05); Compared with RU486 group, the content of PGF2α in the supernatant of adenomyosis cell line of Huayu Zhitong Fang group All of them have significant significance (P <0.05). Conclusion: Huayu Zhitong Fang can down-regulate the secretion of PGE2 and PGF2α in uterine adenomyosis cells, so that the uterus can return to normal systolic function, which may be one of the main mechanisms of analgesia.