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目的 :检测原发性肺癌肿瘤细胞中 DNA含量 ,并探讨其与肺癌 TNM分期、细胞分化程度、细胞增殖活性和凋亡的关系。方法 :应用流式细胞术检测 DNA含量和肿瘤细胞增殖活性 ,TUNEL方法检测肿瘤细胞凋亡。结果 :(1) DNA指数 (DI)的分布范围从 0 .82 9～ 2 .5 14,异倍体肿瘤 41例 (77.4% ) ,DI值和异倍体肿瘤发生率与组织学类型无关 (P >0 .0 5 ) ;(2 ) DI值和异倍体肿瘤发生率与 TNM分期有关 ,随 TNM分期增加而增加 (P <0 .0 5 ) ;(3)低分化肺癌的 DI值高于中分化和高分化肺癌 (P <0 .0 5和 P <0 .0 1) ;(4 )异倍体肿瘤细胞增殖指数 (PI)显著高于二倍体肿瘤 (P <0 .0 1) ,肿瘤细胞凋亡指数 (AI)显著低于二倍体肿瘤 (P <0 .0 1)。结论 :肺癌肿瘤细胞DNA含量与肺癌 TNM分期 ,肿瘤分化程度 ,肿瘤细胞增殖活性和凋亡有关
Objective : To detect DNA content in primary lung cancer tumor cells and to explore its relationship with TNM stage, cell differentiation, cell proliferation activity and apoptosis. Methods : Flow cytometry was used to detect DNA content and tumor cell proliferation activity. Tumor cell apoptosis was detected by TUNEL method. RESULTS: (1) The distribution of DNA index (DI) ranged from 0.82 9 to 2.5.4% in 14 cases (77.4%) of aneuploid tumors. The DI value and the incidence of aneuploid tumors were not related to the histological type. P > 0.05, (2) DI value and aneuploid tumor incidence were associated with TNM stage, and increased with TNM stage (P < 0.05); (3) DI value of poorly differentiated lung cancer was high. Among moderately differentiated and well-differentiated lung cancers (P <0.05 and P <0.01), (4) heteroploid tumor cell proliferation index (PI) was significantly higher than that of diploid tumors (P <0. ) The tumor cell apoptosis index (AI) was significantly lower than that of diploid tumors (P < 0.01). Conclusion: The DNA content of lung cancer cells is related to TNM stage, tumor differentiation, tumor cell proliferation activity and apoptosis.