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目的:研究母鼠在孕前期及孕期经口胃灌十溴联苯醚(brominated diphenyl ethers-209,BDE-209)对出生子鼠数量及体重的影响。方法:48只雌性Wistar大鼠分为4组,通过经口胃灌的方法造成BDE-209[1500mg/(kg·d)]的暴露模型。实验组A(孕前组):自正式进入实验日开始胃灌BDE-209直至雌鼠妊娠,妊娠后改为胃灌纯花生油直至子鼠出生;实验组B(孕期组):自正式进入实验日开始胃灌纯花生油直至雌鼠妊娠,妊娠后改为胃灌BDE-209直至子鼠出生;实验组C(孕前&孕期组):自正式进入实验日开始胃灌BDE-209直至子鼠出生;对照组:自正式进入实验日开始胃灌等量纯花生油直至子鼠出生。子鼠出生后,记录子鼠数量,并称量子鼠出生体重。结果:各组子代大鼠出生数量分别为:(9.60±3.17)、(10.38±2.26)、(11.00±3.83)、(9.38±2.13)只,组间差异无统计学意义(P>0.05);各组子代大鼠出生体重分别为(5.06±0.53)、(4.83±0.68)、(4.80±0.65)、(5.25±0.88)g,组间差异有极显著性(P<0.01),其中孕期胃灌母源性BDE-209使子代大鼠出生体重显著减少(P<0.01),而孕前期胃灌母源性BDE-209则无显著影响(P>0.05),不同时期胃灌交互效应不显著(P>0.05),结论:孕期暴露于BDE-209会造成子鼠出生体重减少,但其对出生子鼠数量的影响尚有待进一步研究。
OBJECTIVE: To study the effects of brominated diphenyl ethers-209 (BDE-209) on the number and body weight of neonatal rats during preconception and pregnancy. Methods: Forty-eight female Wistar rats were divided into 4 groups and their models of BDE-209 [1500 mg / (kg · d)] were induced by oral gastric perfusion. Experimental group A (pre-pregnancy group): From the formal entry into the experimental day, gastric perfusion of BDE-209 until pregnancy in females, gestation after pregnancy to peanut oil until the birth of mice; experimental group B (pregnancy group): formally entered the experimental day Beginning of gastric perfusion of pure peanut oil until pregnancy in females, pregnancy changed to gastric perfusion BDE-209 until the offspring born; experimental group C (pre-pregnancy & pregnancy group): formally entered the experimental day gastric perfusion BDE-209 until the offspring was born; Control group: Since the formal entry into the experimental day began equal volume of peanut oil stomach irrigation until the birth of the offspring. After the birth of the offspring, the number of offspring is recorded and the birth weight of the offspring is measured. Results: The number of offspring rats in each group was (9.60 ± 3.17), (10.38 ± 2.26), (11.00 ± 3.83) and (9.38 ± 2.13) respectively, with no significant difference between the two groups (P> 0.05) (P <0.01). The birth weights of offspring in each group were (5.06 ± 0.53), (4.83 ± 0.68), (4.80 ± 0.65) and (5.25 ± 0.88) g, respectively Prenatal BDE-209 had no significant effect on the birth weight (P <0.01), but had no significant effect on gastric GCN-BDE-209 during pregnancy (P> 0.05) (P> 0.05). Conclusion: Exposed to BDE-209 during pregnancy may result in the loss of birth weight of the offspring, but its effect on the number of born offspring remains to be further studied.