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目的:探讨水飞蓟宾对人卵巢癌HO-8910细胞增殖的抑制作用及其作用机制。方法:HO-8910细胞分为四组:(1)对照组;(2)水飞蓟宾低浓度组;(3)水飞蓟宾中浓度组;(4)水飞蓟宾高浓度组。通过MTT法测定细胞增值率,流式细胞技术检测细胞凋亡情况,Hoechst染色观查细胞核凋亡,Western-blot检测bax及bcl-2表达。结果:细胞增殖检测结果显示,水飞蓟宾低、中、高浓度组的抑制率(83.00±5.51%、65.33±3.48%、56.67±4.37%)与对照组(97.33±4.25%)比较差异具有统计学意义(P<0.05)。流式细胞技术结果显示,水飞蓟宾低、中、高浓度组凋亡细胞比例分别为16.93±2.34%、26.20±2.21%和37.93±1.98%,与对照组(1.43±0.72%)相比差异均有统计学意义(P<0.05)。Hoechst染色结果显示,水飞蓟宾低、中、高浓度组凋亡细胞比例分别为12.56±2.55%、25.73±2.05%和39.14±3.69%,与对照组(0.54±0.67%)相比差异均有统计学意义(P<0.05)。此外,水飞蓟宾可以升高bax基因表达水平,降低bcl-2基因表达平。结论:水飞蓟宾能明显抑制HO-8910细胞增殖,促进细胞凋亡,通过改变凋亡因子表达诱导卵巢癌HO-8910细胞凋亡。
Objective: To investigate the inhibitory effect of silybin on human ovarian cancer cell line HO-8910 and its mechanism. Methods: HO-8910 cells were divided into four groups: (1) control group; (2) silybin low concentration group; (3) silybin mid-concentration group; (4) silybin high concentration group. Cell proliferation was measured by MTT assay, apoptosis was detected by flow cytometry, nuclear apoptosis was detected by Hoechst staining and bax and bcl-2 were detected by Western-blot. Results: The results of cell proliferation assay showed that the inhibitory rates of silybin at low, middle and high concentrations (83.00 ± 5.51%, 65.33 ± 3.48%, 56.67 ± 4.37%) were significantly lower than those of the control group (97.33 ± 4.25%) Statistical significance (P <0.05). The results of flow cytometry showed that the percentage of apoptotic cells in silybin was 16.93 ± 2.34%, 26.20 ± 2.21% and 37.93 ± 1.98%, respectively, compared with that in control group (1.43 ± 0.72%) The differences were statistically significant (P <0.05). The results of Hoechst staining showed that the percentage of apoptotic cells in low, middle and high concentration silybin was 12.56 ± 2.55%, 25.73 ± 2.05% and 39.14 ± 3.69%, respectively, which was significantly lower than that in control group (0.54 ± 0.67%) There was statistical significance (P <0.05). In addition, silybin increased bax gene expression and decreased bcl-2 gene expression. Conclusion: Silybin can obviously inhibit the proliferation and promote the apoptosis of HO-8910 cells, and induce the apoptosis of HO-8910 cells by changing the expression of apoptosis factors.