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应用改进的RAPD方法测定了名贵中药材天麻基因组DNA指纹图谱;通过选择和回收各种天麻种群共有和优良种群特有的DNA片段,加以克隆、测序和生物信息学分析,证明其中5个DNA序列是未报道的,已被美国基因数据库收录,并运用高效液相色谱技术测定了天麻样本的有效成分天麻素含量。运用PCR技术研究了这些DNA序列在9个天麻种群中的分布及其与天麻素含量的关系。结果表明这5个DNA序列在这些天麻种群中的分布各不相同,其中DNA序列1是所研究的全部天麻种群共有、而其伪品没有的特异DNA分子标记;DNA序列2可能与天麻的天麻素含量高有关。这些DNA标记序列可用于天麻的真伪鉴别、品种鉴定和优选优育等。
The improved RAPD method was used to determine the genomic DNA fingerprint of Gastrodia elata Blume. By selecting and recovering the DNA fragments of common and excellent populations of Gastrodia elata Blume populations, cloning, sequencing and bioinformatics analysis showed that 5 DNA sequences were Not reported, has been included in the US genetic database, and the use of high performance liquid chromatography gastrodin determination of the active ingredient gastrodin content. The distribution of these DNA sequences in nine Gastrodia elata populations and their relationship with gastrodin content were studied by PCR. The results showed that these five DNA sequences were distributed differently in these Gastrodia elata populations, of which DNA sequence 1 was common to all Gastrodia elata populations and no specific DNA was found in its counterfeit samples. DNA sequence 2 might be related to Gastrodia elata High content of related. These DNA marker sequences can be used for authentic identification of Gastrodia, identification of species and optimization of fertility and so on.