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在培养基中加入一定浓度NaCl,对小麦F_1花药愈伤组织的耐盐变异体,进行细胞水平上的筛选.采用直接筛选法及逐渐增加盐浓度筛选法,结果表明,后者是提高耐盐变异体频率的有效措施.C_(17)+0.1%NaCl(转移愈伤组织)C_(17)+0.3%NaCl及C_(17)+0.1%NaCl(转移愈伤组织)C_(17)+0.5%NaCl处理的绿苗分化率,较直接筛选法分别提高5.06及15.0倍.在较高(加0.3%NaCl)盐含量的培养基上分化的花粉植株及其后代(H_2、H_3)表现耐盐性较强.获得了在0.4%含盐量土壤上种植能正常或基本正常生长的高耐盐株系3个.
A certain concentration of NaCl was added to the culture medium to screen salt-tolerant variants of anther callus of F_1 in wheat, and the results of the direct screening method and the gradually increasing salt concentration screening method showed that the latter method was to increase salt tolerance (17) + 0.1% NaCl (transfer callus) C_ (17) + 0.3% NaCl and C_ (17) + 0.1% NaCl (transfer callus) C_ (17) +0.5 Compared with the direct screening method, the differentiation rate of green plantlets increased by 5.06 and 15.0 times, respectively.The pollen plants and their offspring (H_2, H_3) differentiated on the medium with higher (plus 0.3% NaCl) salt content showed salt tolerance The results showed that 3 high-salt tolerant lines with normal or almost normal growth on 0.4% saline soil were obtained.