论文部分内容阅读
目的观察益气活血解毒方药中主要活性物质皂苷、黄酮、多糖等组合对脑缺血再灌注损伤的作用及其机制。方法 SD大鼠随机分为假手术组,脑缺血再灌注模型组,皂苷组,黄酮组,多糖组,活性物质组合组,尼莫地平组,每组10只。线栓法制备大脑中动脉局灶性栓塞模型,观察各组对大鼠神经缺损功能的恢复,脑梗死体积,脑指数、脑含水量,缺血侧脑组织超氧化物歧化酶活力、丙二醛含量,白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α等炎症因子的含量,Bcl-2和Bax蛋白表达,尼氏小体数的影响。结果益气活血解毒方药主要活性物质皂苷、黄酮、多糖活性物质组合配伍能显著地减轻中动脉局灶性栓塞模型大鼠的神经功能缺损症状,促进神经功能缺损症状恢复;缩小大鼠中动脉局灶性栓塞后脑组织梗死体积,降低脑梗死体积百分率;减轻脑水肿,降低脑指数和脑含水量;提高缺血再灌注后大鼠脑组织中超氧化物歧化酶活性、降低丙二醛含量;降低缺血侧脑组织白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α等炎症因子的含量;上调Bcl-2蛋白、下调Bax蛋白表达,调节两者比例;抑制尼氏小体数的减少。结论益气活血解毒方药中主要活性物质组合对大鼠脑缺血再灌注引起的损伤具有明显的保护作用。
Objective To observe the effect and mechanism of the combination of saponins, flavonoids, polysaccharides and other active ingredients of Yiqi Huoxue Jiedu Decoction on cerebral ischemia-reperfusion injury. Methods SD rats were randomly divided into sham-operation group, model of cerebral ischemia / reperfusion, saponin group, flavonoid group, polysaccharide group, active substance combination group and nimodipine group. The focal embolism model of middle cerebral artery was established by thread embolization method. The recovery of nerve defect function, volume of cerebral infarction, brain index, brain water content, superoxide dismutase activity in ischemic brain tissue, Aldehyde content, interleukin-1β, interleukin-6 and tumor necrosis factor-α and other inflammatory factors, Bcl-2 and Bax protein expression, Nissl body size. Results The combination of saponin, flavonoids and polysaccharides active ingredients of Yiqi Huoxue Jiedu Decoction could significantly reduce the symptoms of neurological deficits in rats with focal embolism in middle cerebral artery and promote the recovery of symptoms of neurological deficits. Cerebral infarction volume after focal embolization decreased the volume fraction of cerebral infarction; alleviated cerebral edema, decreased cerebral index and brain water content; increased the activity of superoxide dismutase in rat brain after ischemia-reperfusion, decreased the content of malondialdehyde; decreased IL-1β, IL-6 and TNF-α and other inflammatory factors in ischemic brain tissue; up-regulate Bcl-2 protein, down-regulate the expression of Bax protein and regulate the ratio between them; Decrease. Conclusion The main active compound of Yiqi Huoxue Jiedu Decoction has obvious protective effect on the injury induced by cerebral ischemia-reperfusion in rats.