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目的:前期研究证实槲皮素可有效抑制白血病细胞株HL-60细胞增殖,本研究旨在进一步筛选与槲皮素有协同抗白血病作用的药物。方法:不同浓度硼替佐米与HL-60细胞孵育48 h,槲皮素联合硼替佐米孵育HL-60细胞48 h。不同浓度雷利度胺与HL-60细胞孵育48 h,槲皮素联合雷利度胺孵育HL-60细胞48 h。CCK-8法检测细胞存活率。结果:硼替佐米可显著抑制HL-60细胞的增殖。槲皮素联合硼替佐米的IC50值是49.24μmol/L,比单用槲皮素下降13.44μmol/L;等辐射分析法显示两药有协同作用。低浓度雷利度胺(5、10、20、40、80μmol/L)的细胞存活率与对照组比较无统计学差异,高浓度雷利度胺(160、320μmol/L)细胞存活率显著降低;雷利度胺联合槲皮素的IC50值较单用槲皮素无明显下降;等辐射分析法显示两药无协同作用。结论:硼替佐米可显著抑制HL-60细胞的增殖,槲皮素与硼替佐米有协同抑制HL-60细胞增殖的作用。雷利度胺抑制HL-60细胞增殖作用较弱,与槲皮素在抑制HL-60细胞增殖方面无协同作用。
OBJECTIVE: Previous studies confirmed that quercetin can effectively inhibit the proliferation of leukemia cell line HL-60 cells. The aim of this study was to further screen for drugs that have a synergistic anti-leukemia effect with quercetin. Methods: Different concentrations of bortezomib were incubated with HL-60 cells for 48 h. HL-60 cells were incubated with quercetin and bortezomib for 48 h. Different concentrations of lenalidomide were incubated with HL-60 cells for 48 h, and quercetin was combined with lenalidomide to incubate HL-60 cells for 48 h. The CCK-8 assay detects cell viability. RESULTS: Bortezomib significantly inhibited the proliferation of HL-60 cells. The IC50 value of quercetin combined with bortezomib was 49.24 μmol/L, which was 13.44 μmol/L lower than that of quercetin alone; iso-radiometric analysis showed that the two drugs had a synergistic effect. The cell viability of low concentration of lenalidomide (5, 10, 20, 40, and 80 μmol/L) was not statistically different from that of the control group. The survival rate of high-density thalidomide (160, 320 μmol/L) cells was significantly decreased. The IC50 value of lenalidomide combined with quercetin was not significantly lower than that of quercetin alone; isoradiometric analysis showed no synergistic effect of the two drugs. CONCLUSION: Bortezomib can significantly inhibit the proliferation of HL-60 cells, and quercetin and bortezomib can synergistically inhibit the proliferation of HL-60 cells. Lenalidomide inhibited the proliferation of HL-60 cells and had no synergistic effect with quercetin in inhibiting the proliferation of HL-60 cells.