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本文介绍了用高效液相色谱法测定婴儿制剂中维生素 B_1的含量。本法包括以下步骤:(a)取样品,加水溶解;(b)调节 pH 值使蛋白质沉淀;(c)过滤;(d)用阳离子交换柱及萃取系统浓缩维生素 B_1;(e)依次用水及甲醇淋洗离子交换柱,洗脱吸收的维生素 B_1及其它污染物;(f)用甲醇—2 M KCl 缓冲液的混合液萃取维生素 B_1;(g)用高效液相色谱法测定维生素 B_1。
This article describes the determination of vitamin B 1 in infant formula by high performance liquid chromatography. The method comprises the following steps: (a) taking a sample to dissolve in water; (b) adjusting the pH to precipitate the protein; (c) filtering; (d) concentrating the vitamin B_1 by a cation exchange column and an extraction system; Methanol leaching ion exchange column, the absorption of absorbed vitamin B_1 and other contaminants; (f) extraction of vitamin B_1 with a mixture of methanol-2 M KCl buffer; (g) determination of vitamin B_1 by high performance liquid chromatography.