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用测定骨元素含量的方法,观察运动和尼尔雌醇(CCE3)联合应用对去卵巢大鼠骨元素代谢的影响。将健康4月龄雌性SD大鼠40只,用抽签法随机分成5组:正常对照组;假手术组;去卵巢组;去卵巢+CCE3组;去卵巢+CCE3+运动组。去卵巢+CCE3组和去卵巢+CCE3+运动组大鼠于去卵巢手术后第2 d开始给予CCE3灌胃,0.5 mg/(kg.BW),1次/w,持续11周。去卵巢+CCE3+运动组于去卵巢术后第7 d开始运动训练,每周5 d,每天连续匀速跑45 min,16 m/min,跑道倾角0°,持续10周。结果表明,去卵巢组大鼠骨Ca、S、Mg、Zn、Co、Mn等元素含量均明显低于假去卵巢组,骨P含量明显高于假去卵巢组;去卵巢+CCE3组大鼠骨Ca、S、Mg、Zn、Co、Mn等元素含量虽然较去卵巢组明显增加,但仍显著低于假去卵巢组;运动与CCE3联合应用后,去卵巢大鼠骨Ca、S、Mg、Zn、Co、Mn等元素含量均显著回升,骨P含量显著回降,并且基本恢复到对照组水平。提示运动可加强CCE3对抗由于去卵巢引起的大鼠骨元素代谢紊乱。
The content of bone was measured to observe the effect of exercise and nilestriol (CCE3) on bone metabolism in ovariectomized rats. Forty healthy 4-month-old female Sprague-Dawley rats were randomly divided into five groups by random selection: normal control group, sham operation group, ovariectomized group, ovariectomized + CCE3 group, ovariectomized + CCE3 + exercise group. Ovariectomized + CCE3 group and ovariectomized + CCE3 + exercise group were intragastrically administrated with CCE3 at 0.5 mg / (kg.BW) once a day for 2 weeks from the 2nd day after ovariectomy. The ovariectomized + CCE3 + exercise group started exercise training on the 7th day after ovariectomy, and continued to run at a constant speed of 45 min, 16 m / min and runway inclination of 0 ° for 5 days a week for 10 weeks. The results showed that the content of Ca, S, Mg, Zn, Co, Mn and other elements in ovariectomized group were significantly lower than those in sham-ovariectomized group. The content of bone P in ovariectomized group was significantly higher than that in sham-ovariectomized group. Although the contents of Ca, S, Mg, Zn, Co and Mn in bone of ovariectomized rats were significantly higher than those of ovariectomized ovariectomized rats, the content of Ca, S, Mg, Zn, , Zn, Co, Mn and other elements were significantly increased, bone P significantly decreased, and returned to the control group level. Suggesting that exercise can enhance CCE3 antagonism due to ovariectomized rat bone metabolism disorders.