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建立了脂性药物中黄芩甙含量的HPLC分析方法,用H3PO4-Tris缓冲液和甲醇的线性梯度洗脱,少量的黄芩甙和共存于脂性药物中的许多其它成分在C3硅烷键合相上得到满意的分离,方法重现性好,线性范围宽,回收率高.
An HPLC method for the determination of jaundice in lipids was developed. A linear gradient of H3PO4-Tris buffer and methanol was used to elute. A small amount of astragalus and many other components coexistent in lipid drugs were satisfied on the C3 silane bonding phase. The separation method has good reproducibility, wide linear range and high recovery.