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目的探讨柴胡-黄芩水煎液中分离出的5类主要化学成分群的不同配伍与解热作用的相关性,旨在从化学成分群层次上揭示柴胡-黄芩药对解热的药效物质基础。方法依照正交设计L8(27)表将柴胡-黄芩合煎液中分离出的5个化学成分群,依次配制成化学成分群配方1~8。以80μg/kg剂量一次性腹腔注射LPS复制大鼠发热模型;中药各组分别灌胃给予化学成分群配方1~8(剂量均为折合原药材10 g/kg);从注射LPS开始,分别于30、90、150、210、270、330 m in测量体温,连续观察330 m in,计算各组体温变化值ΔT。结果在大鼠发热第2、3峰值处(150、330 m in),配方1、5、6组能够显著降低LPS引起的大鼠体温升高(P<0.05),显示出明显的解热作用。与配方1组相比,各组间不存在显著性差异,但配方5、6组具有更强的解热趋势。各因素方差分析结果表明,化学成分群挥发油部分(B)、黄酮部分(D)和剩余混合物部分(E)在解热效应中发挥重要作用,能协同增强解热效果;而皂苷部分(C)具有明显的拮抗作用;多糖部分(A)对解热作用无显著影响。结论柴胡-黄芩药对中化学成分群的不同配方对发热模型表现出不同的作用,表明各化学成分群的不同配伍与解热作用的相关程度不同。化学成分群BDE为正交设计法优选出的抑制LPS诱导大鼠发热的最佳配方。
Objective To explore the correlation between different compatibility and antipyretic effects of five major chemical constituents isolated from decoction of Radix Bupleuri-Scutellaria baicalensis Georgi, in order to reveal the effect of Radix Bupleurum and Radix Astragali on antipyretic effects from the chemical composition level. Material basis. Methods According to the orthogonal design L8 (27) table, the five chemical components separated from the decoction of Radix Bupleurum and Radix Astragali were sequentially formulated into chemical composition formulas 1-8. A rat model of febrile heat was induced by intraperitoneal injection of LPS at a dose of 80 μg/kg. Rats in each group were given intragastric administration of chemical composition formulas 1 to 8 (10 g/kg as original dose); injections of LPS were started at the same time. Body temperature was measured at 30, 90, 150, 210, 270, and 330 m in, and 330 m in was continuously observed. The temperature change ΔT was calculated for each group. Results At the peak of rats’ febrile peaks 2, 3 and 3 (150 and 330 m in), formula 1, 5 and 6 groups could significantly reduce LPS-induced increases in body temperature in rats (P<0.05), showing significant antipyretic effects. . Compared with the Formula 1 group, there was no significant difference between the groups, but Formula 5 and 6 groups had a stronger tendency to antipyretic. The results of variance analysis of various factors showed that the volatile oil fraction (B), the flavonoid fraction (D) and the remaining mixture fraction (E) of the chemical constituent group play an important role in the antipyretic effect and can synergistically enhance the antipyretic effect; while the saponin moiety (C) has Significant antagonism; polysaccharide fraction (A) had no significant effect on antipyretic effects. Conclusion The different formulas of the middle-chemical composition group of the Radix Bupleuri-Huangxi medicine showed different effects on the fever model, indicating that the different compatibility and antipyretic effects of different chemical composition groups were different. The chemical composition group BDE is the optimal formula for inhibiting LPS-induced fever in rats, which is optimized by the orthogonal design method.