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目的:探讨补充大剂量肌酸对运动大鼠自身肌酸生物合成和体内肌酸代谢的影响。方法:给游泳训练大鼠补充肌酸,测定大鼠肾脏精氨酸-甘氨酸脒基转移酶(AGAT)活性、酶促反应产物胍乙酸含量、骨骼肌肌酸含量、血清肌酸和肌酐含量、血清肌酸激酶(CK)和乳酸脱氢酶(LDH)活性、大鼠游泳力竭时间。结果:补充超大剂量(6 .0g/kg/d)肌酸可引起大鼠腹泻,造成腓肠肌和比目鱼肌湿重显著下降(P <0 .0 5 ) ;补充相当于人体冲击剂量(1 5g/kg/d)和超大剂量肌酸,均可引起运动大鼠肾脏AGAT活性和肝脏胍乙酸含量明显下降(P <0 . 0 1) ;超大剂量补充肌酸引起游泳大鼠血清肌酐含量增加。此外,运动训练能提高肾脏AGAT活性和肝脏胍乙酸含量,促进内源性肌酸的合成。结论:超大剂量补充肌酸可抑制运动机体内源性肌酸的合成。建议实践中不宜超量超时补充肌酸,有必要对人体补充肌酸的冲击剂量(0 . 3g/kd/d或2 0g/d)的健康安全性进行深入研究。
Objective: To investigate the effects of high dose creatine supplementation on creatine biosynthesis and creatine metabolism in rats. Methods: Creatine was supplemented to swimming training rats. The activity of arginine - glycine amidotransferase (AGAT), the content of guanidine acetic acid, the creatine content of skeletal muscle, serum creatine and creatinine, Serum creatine kinase (CK) and lactate dehydrogenase (LDH) activity, rat swimming time to exhaustion. Results: Creatine supplemented with very large dose (6 .0 g / kg / d) of creatine caused diarrhea in rats and caused a significant decrease in the wet weight of gastrocnemius and soleus muscles (P <0.05); supplementation equivalent to human impact dose (15 g / kg / d) and overdose of creatine could cause the activity of AGAT and the content of guanidinium acetate in the liver of exercise rats decreased significantly (P <0.01). The serum creatinine level of swimming rats increased after hyper dose of creatine supplementation. In addition, exercise training can increase renal AGAT activity and liver guanidinium acetate content, and promote the synthesis of endogenous creatine. Conclusion: Hyper-dose creatine supplementation can inhibit the synthesis of endogenous creatine in the motorbodies. Recommendations in practice should not exceed the amount of creatine exaggerated overtime, it is necessary to impact on human body dose of creatine (0. 3g / kd / d or 20g / d) health and safety in-depth study.