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作者采用CBA鼠的脾细胞为效应细胞、抗体致敏的鸡红细胞为靶细胞,结合~51Cr释放试验在扫描电镜下观察了抗体依赖性细胞介导的细胞毒作用(ADCC)的杀伤机制。发现在ADCC过程早期,效应细胞借其表面绒毛状突起与靶细胞膜紧密结合。随后,效应细胞的绒毛状突起插入靶细胞,靶细胞扭曲、变形、损伤。尤其注意到,在与效应细胞绒毛状突起结合的部位,靶细胞膜呈现“卷须样”变化,提示靶细胞的损伤有机械性因素参与。
The authors used CBA mouse spleen cells as effector cells and antibody-sensitized chicken erythrocytes as target cells. The killing mechanism of antibody-dependent cell-mediated cytotoxicity (ADCC) was observed by scanning electron microscopy combined with ~ 51Cr release assay. It was found that in the early stages of ADCC, effector cells are tightly bound to the target cell membrane by their surface villi. Subsequently, villi of effector cells are inserted into the target cells, distorting, deforming, and damaging the target cells. In particular, it was noted that at the site of binding to the villi of effector cells, the target cell membrane showed a “tendril-like” change suggesting the involvement of mechanical factors in the damage of the target cells.