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目的:探讨不同环境适应对大鼠纹状体5-羟色胺(5-HT)及其代谢产物5-羟吲哚乙酸(5-HIAA)和运动能力的影响。方法:成年雄性Wistar大鼠随机分为安静对照组(Q)、高温运动适应组(A)、常温运动适应组(E)和高温暴露适应组(P)4组,每组6只。高温温度设定为38°C,常温温度设定为23°C,相对湿度均为50%~60%。高温运动适应、常温运动适应及热暴露适应时间均为6天,第7天进行高温力竭运动实验,运动结束即刻测试肛温,尾部取血测血乳酸,并测定大鼠纹状体5-HT和5-HIAA,分别测定大鼠腓肠肌乳酸脱氢酶(Lactate dehydrogenase,LDH)和琥珀酸脱氢酶(succinate dehydrogenase,SDH)的活性。结果:高温运动适应组大鼠纹状体5-HT较安静对照组显著增加,高温运动适应组和高温暴露适应组代谢产物5-HIAA较安静对照组均显著上升;高温运动适应组运动时间与高温暴露适应组和常温运动适应组比较均显著增加;高温运动适应组SDH与其余三组比较显著增加,各组LDH无显著性差异;高温运动适应组、常温运动适应组和高温暴露适应组三组肛温均显著增加,高温运动适应组增加较少,组间无显著性差异。结论:6天高温运动适应后大鼠纹状体能耐受更高的5-HT,其代谢产物5-HIAA亦出现了适应性提高。LDH和SDH变化及运动时间结果显示,6天高温运动适应提高了大鼠有氧运动能力。
Objective: To investigate the effects of different environmental adaptation on 5-hydroxytryptamine (5-HT) and its metabolite 5-HIAA and locomotor capacity in rat striatum. Methods: Adult male Wistar rats were randomly divided into 4 groups: quiescent control group (Q), high temperature exercise adaptation group (A), normal temperature exercise adaptation group (E) and hyperthermia exposure adaptation group (P). High temperature is set to 38 ° C, room temperature is set to 23 ° C, relative humidity is 50% to 60%. High temperature exercise adaptation, exercise adaptation at room temperature and heat exposure adaptation time are 6 days, 7 days for high temperature exhaustive exercise test, the end of exercise immediately test rectal temperature, blood from the tail to measure blood lactate, and determination of rat striatum 5- HT and 5-HIAA were used to measure the activities of Lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) in rat gastrocnemius respectively. Results: 5-HT in striatum of rats in hyperthermia adaptation group increased significantly compared with quiet control group, 5-HIAA in hyperthermia adaptation group and hyperthermia exposure adaptation group increased significantly compared with quiet control group. Exercise time of hyperthermia adaptation group was Compared with the other three groups, the SDH in the hyperthermia exercise adaptation group increased significantly, and there was no significant difference in LDH between the high temperature exposure adaptation group and the normal temperature exercise adaptation group; the high temperature exercise adaptation group, the normal temperature exercise adaptation group and the high temperature exposure adaptation group three Group rectal temperature were significantly increased, high-temperature exercise adaptation group increased less, no significant difference between groups. CONCLUSION: After 6 days of hyperthermia, the striatum of rat is able to tolerate higher 5-HT, and its metabolite 5-HIAA also appears to be adaptively increased. LDH and SDH changes and exercise time results show that 6 days of high temperature exercise to improve aerobic exercise capacity in rats.