论文部分内容阅读
目的:建立一个简单、可靠的、可以直接研究单一功能变化和组织形态变化之间联系的大鼠胡须体觉皮层局灶性脑缺血模型。方法:大鼠经10%水合氯醛麻醉后,经右侧顶叶皮层处开颅,在显微镜下结扎大脑中动脉的2～3个分支。应用激光多普勒血流仪检测结扎前、后局部脑血流情况,细胞色素氧化酶染色确定胡须体觉皮层和梗死部位的关系,2,3,5-氧化三苯基四氮唑(TTC)染色检测梗死面积大小。胡须依赖实验检测胡须功能。结果:大脑中动脉主要侧支结扎后,梗死中心及周围局部脑血流明显下降。细胞色素氧化酶染色及TTC染色均示梗死区域位于胡须体觉皮层。局灶性缺血3d,测定梗死面积约占全脑面积的15%。梗死形成后大鼠胡须分辨物体纹理的能力下降。结论:选择性的将大鼠具有单一功能的胡须体觉皮层制作成局灶性脑缺血模型,提供了一个较好的研究功能变化和组织形态变化之间联系的脑缺血动物模型。
OBJECTIVE: To establish a simple and reliable rat model of bearded somatic cortex focal cerebral ischemia that can directly study the connection between single functional changes and histological changes. METHODS: Rats were anesthetized with 10% chloral hydrate and craniotomy was performed via the right parietal cortex. Ligation of 2 to 3 branches of the middle cerebral artery was performed under the microscope. Local cerebral blood flow before and after ligation was detected by laser Doppler flowmetry. The relationship between bearded somatosensory cortex and infarct site was determined by cytochrome oxidase staining. Toxorubicin ) Staining detected infarct size. Beards rely on experiments to test beard functions. Results: Local cerebral blood flow in the center of infarction and surrounding area decreased significantly after the ligation of the middle cerebral artery. Cytochrome oxidase staining and TTC staining showed that the infarct area was located in bearded body somatosensory cortex. Focal ischemia 3d, measured infarct size accounted for about 15% of the whole brain area. The ability of the rat beard to distinguish the texture of an object from declining after infarction has been established. CONCLUSIONS: Selective preparation of a bearded somatosensory cortex with a single function in rats into a model of focal cerebral ischemia provides a better model of cerebral ischemia in animals that is associated with functional changes and histological changes.