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目的 评价微粒子酶免 (MEIA)法检测全血普乐可复浓度的准确性及其在器官移植术后抗排异治疗中的应用。方法 以MEIA法检测 6例心脏移植、2例肝脏移植、2例小肠移植和 7例肾脏移植患者不同时期全血中普乐可复的谷浓度 ,并结合临床情况调整普乐可复的用药剂量。以MEIA法测定高、中、低 3种标准普乐可复全血样品 ,求算MEIA方法学的回收率 ,分析MEIA的准确性及其对临床普乐可复用药的指导作用。结果 在临床 6 83个血样的 472次检测中 ,质控血样检测结果均在控制范围内 ,质控的平均RSD为 (7.7± 2 .0 ) % ,方法回收率为 (96 .4± 7.4) % ,分析过程可在 1h内完成。结论 MEIA法具有快速、简便、专一性强和灵敏度高的特点 ,是一种较理想的普乐可复血药浓度常规检测方法 ,适宜在医院内开展
OBJECTIVE: To evaluate the accuracy of microparticle enzyme immunoassay (MEIA) in the determination of cladogram concentrations in whole blood and its application in anti-rejection therapy after organ transplantation. Methods MEIA was used to detect the concentration of cladocerex in 6 cases of heart transplantation, 2 cases of liver transplantation, 2 cases of small bowel transplantation and 7 cases of kidney transplantation. . MEIA was used to determine the standard high, medium and low of the standard Plelenum - Cola. The recovery rate of MEIA method was calculated. The accuracy of MEIA and its guidance on clinical Pleurum induction were analyzed. Results In the 472 clinical samples of 6 83 blood samples, the quality control blood samples were all within the control range. The average RSD of quality control was (7.7 ± 2.0)% and the recovery rate was (96.4 ± 7.4) %, The analysis process can be completed within 1h. Conclusion MEIA has the characteristics of rapid, simple, specific and high sensitivity, it is an ideal routine test method of concentration of replenishing blood. It is suitable to be carried out in the hospital