几年来,我所采用单倍体育种方法,通过140种处理、80多个组合、5万多个花药的离体无菌培养,诱导出了小麦花粉愈伤组织,并通过愈伤组织,诱导分化出完整的小麦单倍体植株,利用这些植株经过加倍,获得加倍后的种子9个组合,种植二代的两个组合(即“昌潍9号×中阿1号”和“昌潍10号×65(1471—6)”两个组合),种植一代的两个组合,新品系正在选育中。研究说明,利用处于单核中期的小麦花粉接种,能诱导出较多的花粉愈伤组织。而在四分体期接种,没有诱导出花粉愈伤组织。花粉发育期的确定,可采用镜检与观察外 Over the past few years, I have used the method of haploid breeding, through the in vitro sterile culture of 140 treatments, more than 80 combinations, more than 50,000 anthers, the wheat pollen callus was induced and induced by callus The whole wheat haploid plants were differentiated and doubled with these plants to obtain 9 combinations of doubled seeds for planting two combinations of the second generation (ie “Changwei 9 × ZhongA 1” and “Changwei 10 No. × 65 (1471-6) ”two combinations), planting two generations of combinations, the new line is breeding. The research shows that more wheat pollen callus can be induced by using wheat pollen in the mid-monnucleus stage. In quadruplicate inoculation, no pollen callus was induced. Pollen development period can be determined by microscopic examination and observation