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目的:观察补气活血、行气活血代表方补阳还五汤、血府逐瘀汤对急性脑缺血大鼠碱性成纤维细胞生长因子b FGF蛋白表达的影响,探讨不同活血配伍方法对大脑保护作用的差异。方法:采用大脑中动脉线拴法复制局灶性脑缺血再灌注损伤大鼠模型,将大鼠随机分为假手术组、模型组、补气活血组、行气活血组,共4组,每组10只,光镜下观察脑组织病理学变化,免疫组化检测b FGF蛋白的表达。结果:补气活血组、行气活血组b FGF含量明显升高,与模型组比较有显著性差异,补气活血与行气活血组间有显著性差异(P<0.01)。结论:补气活血和行气活血的配伍均能升高脑组织b FGF的含量,但是补气活血作用强于行气活血,对脑组织具有更好的保护作用。
Objective: To observe the effect of Bu Yang Huan Wu Decoction, which is on behalf of qi and activate blood circulation, and Xuefu Zhuyu Decoction on the expression of bFGF protein in basic fibroblast in rats with acute cerebral ischemia Differences in brain protection. Methods: The model of focal cerebral ischemia-reperfusion injury was induced by tethered middle cerebral artery occlusion in rats. The rats were randomly divided into 4 groups: sham-operation group, model group, qi-invigorating and blood- 10 rats in each group. Pathological changes of brain were observed under light microscope. The expression of b FGF protein was detected by immunohistochemistry. Results: The content of b FGF in qi and activating blood circulation group and qi and activating blood circulation group was significantly higher than that in model group, and there was a significant difference between qi and activating blood circulation and qi and activating blood circulation group (P <0.01). CONCLUSION: The combination of qi and activating blood circulation and qi and activating blood circulation can both increase the content of bFGF in brain tissue. However, the effect of qi and activating blood circulation is stronger than that of qi and activating blood circulation and has a better protective effect on brain tissue.