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目的:探讨过氧化氧化还原蛋白(PRDX)ⅠmRNA在小鼠卵泡发育、排卵及卵巢衰老过程中的重要作用。方法:分别选用不同卵巢功能(形成期、性未成熟期、性成熟期、功能退化期)和不同动情周期(动情前期、动情期、动情后期、动情间期)以及促性腺激素预处理不同时间点[注射0.9%氯化钠液(CON)后、注射孕马血清(PMSG)48小时(P48h)后和注射绒促性素(HCG)后4小时(H4h)至48小时(H48h)]C57BL/6j小鼠的卵巢,通过实时定量聚合酶链反应检测PRDXⅠmRNA在卵巢中的表达。结果:①PRDXⅠmRNA在性未成熟期组、性成熟期组和功能退化期组小鼠卵巢中稳定表达,组间比较,差异无统计学意义(P>0.05),但均较形成期组低,差异均有统计学意义(P<0.05)。②在性成熟小鼠不同动情周期间表达比较,差异无统计学意义(P>0.05)。③在促性腺激素预处理卵巢中,与CON组相比,PRDXⅠmRNA在PMSG作用后48小时(P48h组)表达显著升高(P<0.05),而在HCG作用后8小时(H8h组)最低(P<0.05),后于48小时(H48h组)升高(P<0.05)。结论:PRDXⅠmRNA在卵泡发育、排卵和卵巢衰老中可能发挥调控作用。
Objective: To investigate the important role of peroxiredoxin (PRDX) Ⅰ mRNA in mouse follicular development, ovulation and ovarian aging. Methods: The ovarian function (formation stage, immature stage, sexual maturity stage, functional degeneration stage) and different estrous cycle (estrus stage, estrus stage, estrous stage, estrus stage) and gonadotropin pretreatment Point (H48h) 48 hours (P48h) after injection of 0.9% sodium chloride solution (CON) and 4 hours (H48h) after injection of cashmectin (HCG)] C57BL / 6j mice ovaries, PRDX Ⅰ mRNA expression in ovary by real-time quantitative polymerase chain reaction. Results: (1) PRDX Ⅰ mRNA was stably expressed in the ovary of sexual immature group, sexual maturity group and functional degeneration group. There was no significant difference between the two groups (P> 0.05), but both were lower than those in the formation stage All were statistically significant (P <0.05). (2) There was no significant difference in expression between sexually mature mice in different estrus weeks (P> 0.05). (3) Compared with CON group, PRDXⅠmRNA expression was significantly increased in P48G group (P48h group) compared with CON group (P <0.05), and lowest in H8h group (P <0.05) P <0.05), and then increased at 48 hours (H48h group) (P <0.05). Conclusion: PRDX Ⅰ mRNA may play a regulatory role in follicular development, ovulation and ovarian aging.