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本文介绍脾内肝细胞移植技术、检测方法及实验性治疗的新成就。采用胶原酶复循环灌注法从大鼠、犬、猪及猴的肝脏获取活的单个肝细胞,经不同途径注射入脾内。自体移植6～12个月后,新增殖肝细胞形成条索状或铺砖状团块,PAS 和G-6-P 酶染色阳性,且具有白蛋白合成功能及对外源性胆红素和氨的代谢功能,并能摄取~(99m)Tc-HIDA 和排泄吲哚氰绿。脾内网状组织有俘获和保护移植肝细胞不被吞噬细胞吞噬的作用。借自体或同种异体肝细胞移植以形成肝化脾用于治疗先天性高胆红素血症、肝硬化及D-Gal 中毒的实验性肝表已初见成效。
This article describes the intrahepatic liver cell transplantation technology, detection methods and experimental treatment of new achievements. Live single hepatocytes were harvested from the liver of rats, dogs, pigs and monkeys by collagenase perfusion, and injected into the spleen through different routes. After 6 to 12 months of autotransplantation, newly proliferating hepatocytes formed cord-like or brick-like masses with positive staining for PAS and G-6-P enzymes and albumin synthesis as well as sensitivity to exogenous bilirubin and ammonia Metabolic function, and can take ~ (99m) Tc-HIDA and excretion indocyanine green. Spleen reticulated tissue capture and protect graft liver cells are not phagocytic phagocytic role. Experimental liver plaque autologous or allogeneic liver cell transplantation to form hepaticized spleen for the treatment of congenital hyperbilirubinemia, cirrhosis and D-Gal poisoning has achieved initial success.