论文部分内容阅读
为了研究铅镉联合暴露对大鼠肝功能的影响以及α-硫辛酸的保护作用,试验以300 mg/L铅(Pb)、50 mg/L镉(Cd)单独或联合(Pb+Cd)采用自由饮水方式染毒SD大鼠,α-硫辛酸保护组在Pb、Cd自由饮水的同时按体重以50 mg/kgα-硫辛酸灌胃,12周后测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、血清碱性磷酸酶(ALP)活性及总蛋白(TP)、白蛋白(ALB)的含量。结果表明:与对照组相比,铅组、镉组ALT活性极显著升高(P<0.01),AST和ALP活性极显著下降(P<0.01),镉组TP和ALB含量显著或极显著下降(P<0.05或P<0.01),铅组TP和ALB含量无显著性差异,铅镉联合组ALT和AST活性极显著升高(P<0.01),ALP活性、TP和ALB含量无显著性差异;与铅、镉单独染毒组相比,铅镉联合组ALT、AST活性极显著升高(P<0.01),ALP活性、TP和ALB含量无显著性差异;与相应染毒组相比,α-硫辛酸保护组大鼠肝功能各项指标均有所恢复,部分具有统计学意义(P<0.05)。说明铅、镉单独或联合暴露对大鼠肝功能有损伤作用,α-硫辛酸可在一定程度上降低铅、镉单独或联合引起的肝功能损伤。
In order to study the effect of Pb and Cd combined exposure on liver function and the protective effect of α-lipoic acid, the experiment was conducted with 300 mg / L Pb and 50 mg / L Cd alone or in combination (Pb + Cd) Free-water exposure to SD rats, α-lipoic acid protection group in the free drinking of Pb, Cd by body weight at 50 mg / kg α-lipoic acid gavage, 12 weeks after the determination of serum alanine aminotransferase (ALT ), Aspartate aminotransferase (AST), serum alkaline phosphatase (ALP) activity, total protein (TP) and albumin (ALB) The results showed that compared with control group, ALT activity in lead group and cadmium group were significantly increased (P <0.01), AST and ALP activity significantly decreased (P <0.01), and TP and ALB in cadmium group significantly decreased (P <0.05 or P <0.01). The contents of TP and ALB in the lead group showed no significant difference. The activities of ALT and AST in the lead group were significantly increased (P <0.01), and there was no significant difference in the activities of ALP, TP and ALB (P <0.01). There was no significant difference in ALP activity and TP and ALB content between the two groups (P <0.05). Compared with the control group, the activities of ALT and AST were significantly increased The indexes of liver function in the a-lipoic acid protection group were recovered, some of them were statistically significant (P <0.05). These results indicate that lead and cadmium, alone or in combination, can impair liver function in rat. Alpha-lipoic acid can reduce the damage of liver function induced by lead and cadmium alone or in combination.