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目的:观察通痹合剂对雄性大鼠生殖器官的毒性影响。方法:27只成熟雄性SD大鼠,随机分成3组,每组9只:对照组喂服生理盐水10mL.kg-1.d-1,合剂1组喂服合剂30g.kg-1.d-1,合剂2组喂服合剂10g.kg-1.d-1,60天后处死。检测睾丸系数、精子密度、形态、活力等。光镜观察睾丸和附睾组织的病理变化。结果:与对照组比较,合剂1组睾丸系数、精子密度显著下降(P<0.01),合剂2组无统计学差异(P>0.05)。给药组精子形态异常,活动力丧失。光镜下合剂1组睾丸组织严重损伤,合剂2组睾丸组织几乎无损伤。结论:通痹合剂具有明显的抗雄性生育作用,其影响程度呈剂量-效应关系。
Objective: To observe the toxic effects of tongbi mixture on reproductive organs of male rats. Methods: Twenty-seven male Sprague-Dawley rats were randomly divided into 3 groups with 9 rats in each group: the control group was given normal saline 10mL.kg-1.d-1, the mixture of 1 group was fed with 30g.kg-1.d- 1, the mixture of 2 groups fed with a mixture of 10g.kg-1.d-1, 60 days after sacrifice. Testis coefficient, sperm density, morphology, vitality and so on. Light microscope observation of testicular and epididymal tissue pathological changes. Results: Compared with the control group, testis coefficient and sperm density decreased significantly in group 1 (P <0.01), and there was no significant difference in group 2 (P> 0.05). The administration group sperm morphology abnormalities, loss of activity. Under the light microscope, testicle tissue of group 1 was severely damaged, and testicle tissue of group 2 was almost no damage. Conclusion: Tongbi Mixture has significant anti-male reproductive effects, and its effect is dose-effect relationship.