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目的探讨急性放射性皮肤溃疡形成早期b-FGF、VEGF及其受体的表达和意义。方法以50Gy60Coγ射线单次局部照射建立急性放射性皮肤溃疡动物模型,以手术方法建立单纯皮肤伤口动物模型,采用免疫组化、原位杂交等方法检测伤口及溃疡组织内b-FGF、VEGF及其受体的表达。结果创伤组伤后5～10d,肉芽组织内b-FGF、VEGF表达呈强阳性;照射后21～28d,放射性溃疡肉芽组织内b-FGF、VEGF表达呈阳性,但较创伤后5～10d明显减弱。结论辐射诱导的b-FGF、VEGF表达减弱与急性放射性皮肤溃疡愈合延迟有关。
Objective To investigate the expression and significance of b-FGF, VEGF and their receptors in the early stage of acute radiation-induced skin ulcer. Methods The animal model of acute radiation-induced skin ulcer was established by a single local irradiation with 50Gy60Coγ-rays. The skin wounds were established by surgical methods. The expressions of b-FGF, VEGF and VEGF were detected by immunohistochemistry and in situ hybridization Body expression. Results The expression of b-FGF and VEGF in granulation tissue was strongly positive from 5 to 10 days after wounding. The expression of b-FGF and VEGF in granulation tissue of radiated ulcer was positive from 21 to 28 days after irradiation, but was significantly higher than that from 5 to 10 days after wounding Weakened. Conclusion The decrease of radiation-induced b-FGF and VEGF expression is associated with delayed healing of acute radiation-induced skin ulcers.