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目的 :探讨聚合酶链反应 (PCR)在流感嗜血杆菌 (Hi)肺炎诊断中的应用价值。方法 :应用 Hi种特异性PCR和 b型 Hi(Hib)特异性 PCR及选择性 Hi培养基 ,对 83例婴幼儿肺炎急性期鼻咽深部分泌物 (NPA)和 5 1份血标本 ,及 37份健康婴幼儿咽拭子标本进行检测。结果 :83份 NPA标本培养阳性 2 0份 (2 4 .1% ) ,Hi- PCR阳性 36份 (43.4 % ) ,其中 Hib- PCR阳性 19份 (2 2 .9% ) ,Hi- PCR阳性而 Hib- PCR阴性 17份 (2 0 .5 % ) ;5 1份血标本培养均阴性 ,Hi- PCR和 Hib- PCR均阳性 6份 ;37份对照咽拭子培养阳性 3份 (8.1% ) ,Hi- PCR阳性 6份 (16 .2 % ) ,Hib-PCR均阴性。结论 :健康婴幼儿咽部不定型 Hi携带率较高 ,肺炎 NPA标本以 Hib- PCR检测为宜。
Objective: To investigate the value of polymerase chain reaction (PCR) in the diagnosis of Haemophilus influenzae pneumonia. Methods: Eighty-three nasopharyngeal secretions (NPA) and 51 blood samples were collected from 83 infants with acute pneumonia and 51 blood samples by Hi-specific PCR, b-type Hi (Hib) specific PCR and selective Hi medium Healthy baby throat swab specimens were tested. RESULTS: Totally 20 (21.4%) were positive in 83 NPA specimens and 36 (43.4%) were Hi-PCR positive specimens, of which 19 were positive for Hib-PCR (22.9%) and positive for Hi-PCR Hib-PCR-negative 17 (20.5%); 5 blood samples were negative in culture, Hi-PCR and Hib-PCR were 6 positive; 37 control pharyngeal swab culture positive 3 (8.1% Hi-PCR positive 6 (16.2%), Hib-PCR were negative. Conclusion: The prevalence of unsteady Hi in healthy children and infants is higher than that in NPA specimens detected by Hib-PCR.