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为了研究过氧化物酶、过氧化氢、苯丙氨酸解氨酶和α-淀粉酶之间的关系以及这些酶与油菜生长的关系,对十字花科(Cruciferae)植物油菜不同器官(根、茎、花、叶)的过氧化物酶、α-淀粉酶、苯丙氨酸解氨酶的活性以及可溶性蛋白和过氧化氢含量进行测定;同时采用聚丙烯酰胺凝胶活性电泳方法,对过氧化物酶及α-淀粉酶同工酶谱进行了分析.结果表明,不仅油菜不同器官的过氧化物酶、α-淀粉酶、苯丙氨酸解氨酶的活性、可溶性蛋白和过氧化氢含量存在着差异,而且其过氧化物酶和α-淀粉酶同工酶谱条带在不同器官之间也存在着差异.
In order to study the relationship between peroxidase, hydrogen peroxide, phenylalanine ammonia-lyase and alpha-amylase and the relationship between these enzymes and the growth of rapeseed, different organs (roots, Stems, flowers, leaves) peroxidase, α-amylase, phenylalanine ammonia-lyase activity and soluble protein and hydrogen peroxide were determined; at the same time by polyacrylamide gel electrophoresis, Oxidase and α-amylase isozymes.The results showed that not only the activities of peroxidase, α-amylase, phenylalanine ammonia-lyase, soluble protein and hydrogen peroxide in different organs of rape There are differences in content, and its peroxidase and alpha-amylase isozyme bands in different organs are also different.