对不同背景抗HBc(+)259份血清,采用聚合酶链反应(PCR)及生物素(B:0)两种方法检测HBVDNA结果进行比较。结果:抗HBc(+)/HBsAg(+)、抗HBc(+)/HBeAg(+)及抗HBc(+)/抗HBs(+)血清,前者检测阳性率分别为100%、100%、7.14%;后者分别为38.46%、51.63%、0。证实PCR检测血清中微量HBVDNA最敏感、特异性最强。另对HBsAg(-)血清应用PCR检测单项抗HBc(+)血清中HBVDNA阳性率43.48%,而单项抗HBcIgM(+)、抗HBcIgA(+)及抗HBcIgE(+)均为100%。为单项不同型抗HBc(+)血清中HBVDNA存在提供了参数。 HBsAg was detected by polymerase chain reaction (PCR) and biotin (B: 0) in 259 serum samples with different background levels. Results: The positive rates of anti-HBc (+) / HBsAg (+), anti-HBc (+) / HBeAg (+) and anti-HBc (+) / anti-HBs (+) serum were 100%, 100%, 7.14 %; The latter were 38.46%, 51.63%, respectively. Confirmed by PCR detection of serum trace HBVDNA the most sensitive and most specific. The positive rate of HBVDNA in single anti-HBc (+) serum was 43.48% detected by PCR with HBsAg (-) serum, while the single anti-HBcIgM (+), anti-HBcIgA (+) and anti-HBcIgE (+) were all 100%. Provided parameters for the presence of HBVDNA in a single different anti-HBc (+) serum.