论文部分内容阅读
目的研究妊娠期肝内胆汁淤积症(ICP)患者Th1和Th2型细胞免疫反应的变化,从免疫学角度探讨ICP发病机制。方法采用流式细胞术,分别检测了20例重度ICP患者与20例正常晚期妊娠妇女的外周血单个核细胞和T细胞中Th1和Th2细胞所占的比例。结果重度ICP组外周血单个核细胞中IFN-γ+细胞比例为17.82%±3.43%,明显高于正常晚期妊娠组12.66%±2.34%(P<0.01),而IL-4所占的比例为1.17%±0.26%,低于正常晚期妊娠组1.37%±0.30%,差异有显著性(P<0.05);重度ICP组PB-MC中细胞因子Th1/Th2比率为16.00±4.66,显著高于正常晚期妊娠组9.89±2.82(P<0.01);重度ICP组外周血中T细胞分泌的Th1细胞比例为14.37%±1.38%,明显高于正常晚期妊娠组10.66%±1.18%(P<0.01),而Th2细胞比例1.05%±0.19%,低于正常晚期妊娠组1.24%±0.29%,差异有显著性(P<0.01);重度ICP组T细胞Th1/Th2比率为13.99±2.46,显著高于正常晚期妊娠组8.94±1.84(P<0.01)。结论重度ICP患者与正常晚期妊娠妇女比较,其体内表现为Th1型免疫反应增强,而Th2型免疫反应减弱。体内Th1/Th2细胞平衡紊乱可能与妊娠期肝内胆汁淤积症发生相关。
Objective To study the changes of Th1 and Th2 cell immune responses in patients with intrahepatic cholestasis of pregnancy (ICP) and to explore the pathogenesis of ICP from an immunological perspective. Methods Flow cytometry was used to detect the proportion of Th1 and Th2 cells in peripheral blood mononuclear cells and T cells in 20 severe ICP patients and 20 normal pregnant women. Results The percentage of IFN-γ + cells in peripheral blood mononuclear cells in severe ICP group was 17.82% ± 3.43%, significantly higher than that in normal late pregnancy group (12.66% ± 2.34%, P <0.01), while the proportion of IL-4 1.17% ± 0.26%, which was lower than 1.37% ± 0.30% in normal advanced pregnancy group (P <0.05). The Th1 / Th2 ratio in PBMC of severe ICP group was 16.00 ± 4.66, which was significantly higher than that of normal 9.89 ± 2.82 in late pregnancy group (P <0.01). The proportion of Th1 cells secreted by T cells in severe ICP group was 14.37% ± 1.38%, which was significantly higher than that in normal late pregnancy group (10.66% ± 1.18%, P <0.01) While Th2 cell ratio was 1.05% ± 0.19%, which was lower than that of normal late pregnancy group (1.24% ± 0.29%, P <0.01). The Th1 / Th2 ratio of T cells in severe ICP group was 13.99 ± 2.46, which was significantly higher than that of normal The late pregnancy group 8.94 ± 1.84 (P <0.01). Conclusion Severe ICP patients compared with normal late pregnant women, the performance of their Th1-type immune response in vivo, while the Th2-type immune response weakened. In vivo Th1 / Th2 balance disorders may be related to the occurrence of intrahepatic cholestasis of pregnancy.