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目的:研究周期性张应变对面颌肌细胞Na+/K+-ATPaseα亚单位蛋白表达的影响以确定其作用及机制。方法:在建立面颌肌细胞的力学刺激--细胞体外培养模型的基础上,采用Western blot法分析周期性张应变对Na+/K+-ATPaseα1和α2亚单位蛋白表达的影响,加力组分别给予1、2、12、24和48h的力学刺激,施加力值为15%的细胞形变,频率为10cycles/min。以静态组为对照组。对照组及实验组各包含4个实验样本。Western blot检测Na+/K+-ATPaseα1和α2亚单位蛋白的表达。结果:α1亚单位的蛋白表达量除加力1h组与对照组之间、加力24 h与48 h之间无统计学差异外,其余各组之间以及各组与对照组之间均有显著的统计学意义。α2亚单位蛋白表达量除加力24 h与48 h组之间无统计学差异外,其余各组之间以及各组与对照组之间均有显著的统计学意义。结论:在一定时间范围内,周期性张应变可刺激α1和α2亚单位蛋白表达增加,随作用时间的延长蛋白表达受抑制。提示在肌能力的刺激下,面颌肌细胞的相关酶蛋白的功能及表达将发生适应性改建,但其功能亚基的调控机制可能不同。这为选择不同的方法和手段进行临床干预提供了理论依据,因而具有重要的参考意义。
Objective: To study the effect of cyclic tensile strain on the expression of Na + / K + -ATPase alpha subunit protein in facial muscle cells of maxillofacial muscle to determine its mechanism and action. Methods: Based on the establishment of the model of mechanical stimulation of facial maxillary muscle cells cultured in vitro, Western blot was used to analyze the effect of cyclic tensile strain on the protein expressions of Na + / K + -ATPaseα1 and α2 subunits. Mechanical stimulation at 1, 2, 12, 24, and 48 h applied a cell deformation force of 15% at a frequency of 10 cycles / min. The static group as control group. Control and experimental groups each contain 4 experimental samples. Western blot was used to detect the expression of Na + / K + -ATPaseα1 and α2 subunit proteins. Results: There was no significant difference in α1 subunit protein expression between 1 h group and control group, 24 h and 48 h after adding force, and between the other groups and the control group Significant statistical significance. α2 subunit protein expression in addition to 24 h and 48 h group was no significant difference between the addition of force, the other groups and between groups and the control group were statistically significant. CONCLUSION: Periodic tensile strain can stimulate the expression of α1 and α2 subunits in a certain time range, and the expression of protein is inhibited with prolongation of time. It is suggested that the function and expression of related enzyme proteins of facial maxillary muscle cells will be adaptively modified under the stimulation of muscle function, but the regulatory mechanism of functional subunits may be different. This provides a theoretical basis for choosing different methods and means for clinical intervention, and therefore has important reference value.