【摘 要】
：
目的基于蛋白质组学以秀丽隐杆线虫为模式生物探究鸦胆子油的急毒性及抗肿瘤机制。方法提取、制备鸦胆子油乳,稀释成不同浓度后加入96孔板,置于恒温摇床中分别培养1 d观察线虫的死亡比率;培养5 d后观察野生型线虫的比率,分析鸦胆子油乳的急毒性与抗肿瘤活性。提取线虫总蛋白,运用串联质谱标签定量蛋白质组学研究药物干预前后的差异蛋白情况。结果鸦胆子油的毒性较小,浓度为25 mg·L-1时,线虫的死亡率为46%;与对照组相比,给药组均对Ras基因突变的线虫多阴门表型有不同程度的抑制作用,且随着浓度
【基金项目】
：
国家自然科学基金(81760789,82074419),甘肃中医药大学科创基金重点项目。
论文部分内容阅读
目的基于蛋白质组学以秀丽隐杆线虫为模式生物探究鸦胆子油的急毒性及抗肿瘤机制。方法提取、制备鸦胆子油乳,稀释成不同浓度后加入96孔板,置于恒温摇床中分别培养1 d观察线虫的死亡比率;培养5 d后观察野生型线虫的比率,分析鸦胆子油乳的急毒性与抗肿瘤活性。提取线虫总蛋白,运用串联质谱标签定量蛋白质组学研究药物干预前后的差异蛋白情况。结果鸦胆子油的毒性较小,浓度为25 mg·L-1时,线虫的死亡率为46%;与对照组相比,给药组均对Ras基因突变的线虫多阴门表型有不同程度的抑制作用,且随着浓度
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