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目的探讨血管生成素-2(Angiopoietin-2,Ang-2)对人结肠癌细胞SW1116增殖的影响及其机制。方法用不同浓度的Ang-2处理SW1116细胞,MTT法检测其对SW1116细胞增殖的影响;将SW1116细胞分为正常对照、无血清DMEM、Ang-2(1.2 mg/L)及PI3K/Akt阻断剂LY294002(10μmol/L)+Ang-2组,作用24 h后,MTT法检测LY294002对SW1116细胞增殖的影响,Western blot分析Tie-2、PI3K和Akt蛋白的表达。结果 Ang-2在1.2 mg/L时,对SW1116细胞增殖的影响最显著;LY294002能有效抑制由Ang-2引起的SW1116细胞的增殖;Ang-2组与无血清DMEM组比较,Tie-2的表达略有上升,但差异无统计学意义(P>0.05),Akt蛋白的表达显著增强(P<0.01),而PI3K蛋白的表达显著降低(P<0.01),LY294002+Ang-2组中3种蛋白的表达与Ang-2组相比,均明显降低(P均<0.01)。结论 Ang-2能促进SW1116细胞增殖,LY294002可抑制由Ang-2引起的SW1116细胞的增殖,其机制可能与Tie-2/PI3′-kinase/Akt调节的信号通路有关。
Objective To investigate the effect of angiopoietin-2 (Ang-2) on the proliferation of human colon carcinoma cell line SW1116 and its mechanism. Methods SW1116 cells were treated with different concentrations of Ang-2, and the effects of Ang-2 on the proliferation of SW1116 cells were detected by MTT assay. SW1116 cells were divided into normal control, serum-free DMEM, Ang-2 (1.2 mg / L) and PI3K / Akt The effect of LY294002 on the proliferation of SW1116 cells was detected by MTT assay and the expressions of Tie-2, PI3K and Akt proteins were detected by Western blotting after treatment with LY294002 (10μmol / L) + Ang-2 for 24 hours. Results Ang-2 at 1.2 mg / L had the most significant effect on the proliferation of SW1116 cells. LY294002 could effectively inhibit the proliferation of SW1116 cells induced by Ang-2. Compared with serum-free DMEM group, (P <0.01), while the expression of PI3K protein was significantly decreased (P <0.01). In LY294002 + Ang-2 group, the expression of Akt protein was significantly increased (P <0.01) Compared with Ang-2 group, the expression of seed protein was significantly decreased (all P <0.01). Conclusions Ang-2 can promote the proliferation of SW1116 cells. LY294002 can inhibit the proliferation of SW1116 cells induced by Ang-2, which may be related to the regulation of Tie-2 / PI3’-kinase / Akt signaling pathway.