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同步荧光光谱反映出F同时随λex和λem的变化情况,因而得到广泛的应用。本文探索了V_(R1)和V_(B6)双组份体系的固定波长差同步三维荧光光谱的数学模拟方法,并对数学模式进行了实验验证。1 固定波长差同步三维荧光光谱的数学模拟方法 固定波长差同步三维荧光光谱的数学表达式:单组份体系为F=KCf(λ)f(△λ);多组份体系为F=∑KiCifi(λ)fi(△λ);其中λ变量包含着λ_(ex)和λ_(em)两个参数;λ值应满足
Synchronous fluorescence spectrum reflects the change of F with both λex and λem, and thus has been widely used. This paper explores the mathematical simulation method of fixed-wavelength difference 3D synchronous fluorescence spectroscopy in V_ (R1) and V_ (B6) two-component systems. The mathematical model is also verified by experiments. A fixed wavelength difference synchronous three-dimensional fluorescence spectroscopy mathematical simulation method Fixed wavelength difference synchronous three-dimensional fluorescence spectrum mathematical expression: one-component system F = KCf (λ) f (△ λ); multicomponent system F = ΣKiCifi (λ) fi (Δλ); where the λ variable contains two parameters λ_ (ex) and λ_ (em); the value of λ should satisfy