Objective To extract the high-quality total RNA from the seeds of Viola yedoensis. Method The seeds of V. yedoensis were embedded in both self-made embedding medium and synthetic glue,then sectioned with frozen section machine for extracting RNA; the concentration,purity and integrity of yielded RNA were detected for comparing two embedding methods. Results The concentrations RNA extracted by self-made embedding medium and synthetic glue were determined 3. 42 and 1. 04 μg/μL respectively. Agarose gel electrophoresis apparently detected two distinct bands of 28s rRNA and 18s rRNA,with brightness ratio of 28S rRNA to 18S rRNA higher than 1. However, the total RNA extracted by the synthetic glue embedding medium were smeared. Conclusion Using self-made frozen section embedding medium method to extract total RNA from seeds of V. yedoensis method can well meet the requirements of most molecular biological experiments. It is a cost-saving,effective and simple method for high-quality RNA extraction. Objective To extract the high-quality total RNA from the seeds of Viola yedoensis. Method The seeds of V. yedoensis were embedded in both self-made embedding medium and synthetic glue, then sectioned with frozen section machine for extracting RNA; the concentration, purity and integrity of yielded RNA were detected for comparing two embedding methods. Results The concentrations RNA extracted by self-made embedding medium and synthetic glue were determined 3. 42 and 1. 04 μg / μL respectively. Agarose gel electrophoresis apparently detected two distinct bands of 28s rRNA and 18s rRNA, with brightness ratio of 28S rRNA to 18S rRNA higher than 1. However, the total RNA extracted by the synthetic glue embedding medium were smeared. Conclusion Using self-made frozen section embedding medium method to extract total RNA from seeds of V. yedoensis method can well meet the requirements of most molecular biological experiments. It is a cost-saving, effective and simple method for high-quality RNA ex traction.