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目的:建立测定附子理中丸中新乌头碱、乌头碱和次乌头碱的液相色谱-质谱分析方法。方法:采用PolarisC18-A(50mm×2.0mm,5μm)色谱柱,流动相为甲醇-水(90∶10),流速0.2mL·min-1,采用电喷雾电离源正离子质谱检测。结果:新乌头碱、乌头碱和次乌头碱浓度分别在0.232～1.392μg.mL-1(r=0.9997)、0.102～0.612μg.mL-1(r=0.9994)和0.241～1.446μg.mL-1(r=0.9996)范围内线性关系良好,平均回收率(n=6)分别为98.7%(RSD=2.9%)、98.6%(RSD=3.0%)和98.1%(RSD=3.2%)。结论:该法是一种快速、灵敏、准确的分析方法,可以为附子理中丸的质量控制提供科学依据。
Objective: To establish a liquid chromatography-mass spectrometry method for the determination of mesaconitine, aconitine and hypaconitine in aconite Lizhong pills. Methods: Polaris C18-A (50mm × 2.0mm, 5μm) column was used. The mobile phase was methanol-water (90:10) and the flow rate was 0.2mL · min-1. Results: The concentrations of mesaconitine, aconitine and hypaconitine were 0.232-1.392μg.mL-1 (r = 0.9997), 0.102-0.612μg.mL-1 (r = 0.9994) and 0.241-1.446μg (n = 6) were 98.7% (RSD = 2.9%), 98.6% (RSD = 3.0%) and 98.1% (RSD = 3.2% ). Conclusion: This method is a rapid, sensitive and accurate analytical method, which can provide a scientific basis for the quality control of Fuzi Li Zhong Pills.