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目的:探讨建立寒凝证动物模型的最佳温度条件。方法:将50只雌性SD大鼠随机分为A、B、C、D、E 5组,每组10只,A组为对照组,B、C组为-15℃冷冻组,D、E组为-25℃冷冻组,各组均冷冻5 d,每天冷冻4 h。C、E组同样冷冻后,正常喂养5 d。观察大鼠体征和体重,在冷冻结束后第1天和第5天取血检测血液流变学指标。结果:与A组比较,B、C、D、E组大鼠体征变化评分均明显升高,P<0.05;与A组比较,B组和D组冷冻期内大鼠体重下降,P<0.05,E组冷冻结束后体重增加较少,P<0.05;与A组比较,B组与D组全血还原黏度高切明显升高,P<0.05,红细胞刚性指数有升高趋势,但未见统计学差异。结论:每天-25℃、4 h,连续冷冻5 d,可建立寒凝证模型,结合整体体征评分及血液流变学指标评价,符合中医寒凝证候特点。
Objective: To explore the best temperature condition for establishing animal model of cold syndrome. Methods: Fifty female SD rats were randomly divided into A, B, C, D and E 5 groups, with 10 rats in each group. A group was control group, B and C groups were frozen at -15 ℃, D and E groups In the -25 ℃ freezing group, each group was frozen for 5 days and frozen for 4 hours every day. Groups C and E were also fed for 5 days after the same freezing. The signs and body weights of rats were observed. Blood samples were collected on the first day and the fifth day after freezing to measure the hemorrheological indexes. Results: Compared with group A, the score of signs of rats in groups B, C, D and E were significantly increased (P <0.05). Compared with group A, the body weight of rats in groups B and D decreased significantly during freezing (P <0.05) , Group E less weight gain after freezing, P <0.05; compared with Group A, B and D group significantly reduced the viscosity of the whole blood significantly increased, P <0.05, RBC Rising trend, but no Statistical differences. CONCLUSION: Cold-condensing syndrome model can be established by freezing at -25 ℃, 4 h every day for 5 days. Combined with the evaluation of overall signs and hemorheological indexes, it conforms to the characteristics of traditional Chinese medicine syndromes.