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目的:优选白花蛇舌草黄酮类成分的提取工艺,为该药材的资源开发提供参考。方法:以乙醇体积分数、乙醇用量及提取时间为自变量,芦丁、异槲皮苷、槲皮苷提取量的总评“归一值”为因变量,通过Design-Expert 8.0软件对自变量各水平进行多元线性回归和二项式拟合,利用效应面法优选提取工艺并进行预测分析。采用HPLC-DAD测定芦丁、异槲皮苷、槲皮苷含量,色谱条件为流动相乙腈(A)-0.05%磷酸溶液(B)梯度洗脱,检测波长254 nm。结果:最佳提取工艺为加14倍量73%乙醇回流提取2次,每次1.5 h;芦丁、异槲皮苷、槲皮苷平均提取量分别为2.529,0.435,0.215 mg·g-1,总评“归一值”实测值0.922 7与预测值偏差-1.24%。结论:优化的提取工艺稳定可行,预测准确度高,为白花蛇舌草总黄酮的制剂开发提供参考。
Objective: To optimize the extraction process of flavonoids from Hedyotis diffusa Willd. And provide references for the resource development of this herb. Methods: The independent variables of rutin, isoquercitrin and quercitrin were taken as the independent variables, the volume fraction of ethanol, the amount of ethanol and the extraction time were taken as independent variables. The “normalized value” The variables were multivariate linear regression and binomial fitting at each level, and the effect of surface extraction method was optimized and the prediction analysis was conducted. The contents of rutin, isoquercitrin and quercitrin were determined by HPLC-DAD. The mobile phase consisted of gradient elution with acetonitrile (A) -0.05% phosphoric acid solution (B). The detection wavelength was 254 nm. Results: The best extraction process was extraction of 14 times the amount of 73% ethanol reflux extraction twice, each 1.5 h; rutin, isoquercitrin, quercitrin average extraction were 2.529,0.435,0.215 mg · g-1 , The total rating “normalized value ” measured value of 0.922 7 deviation from the forecast value of -1.24%. Conclusion: The optimized extraction process is stable and feasible, and the prediction accuracy is high. It provides a reference for the formulation development of flavonoids from.