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1991年7月下旬至10月上旬,辽宁东沟县沿海出现杂色蛤大批死亡,从病杂色蛤体内分离到病原菌,经人工感染试验得到证实。病原菌为革兰氏阴性短杆菌,以极生单鞭毛运动,在TCBS琼脂平板上形成黄色大菌落,在固体培养基上能游动。发酵葡萄糖产酸不产气,精氨酸—碱反应阴性,赖氨酸、鸟氨酸脱羧阳性,对弧菌抑制剂0/129(150μg)敏感,被鉴定为溶藻弧菌(Vibrio alginolyticus)。通过回接试验证明此溶藻弧菌使杂色蛤死亡。
From late July to early October 1991, a large number of variegated clams appeared on the coast of Donggou County, Liaoning Province. Pathogenic bacteria were isolated from the variegated clams and confirmed by artificial infection test. Pathogenic bacteria Gram-negative Brevibacterium, with polar flagella movement, the formation of yellow colony on TCBS agar plate, in the solid medium to swim. Fermented glucose did not produce acid, arginine-base reaction was negative, lysine, ornithine decarboxylase positive, Vibrio alginolyticus 0/129 (150μg) sensitive, was identified as Vibrio alginolyticus . Through the back-connection test, it proved that V. alginolyticus caused the death of variegated clams.