目的　研究西红花酸对不同自由基发生系统诱导的 DNA损伤的保护作用。方法　通过葡聚糖凝胶、TBA反应、紫外碱基吸收来检测西红花酸对 DNA的保护作用。结果　 DNA被 OH·进攻损伤后 ,碱基在 2 60 nm处紫外吸收下降 ,DNA脱氧核糖氧化破坏 ,其氧化产物与 TBA反应在 5 3 2 nm处出现特征吸收 ,DNA的链结构破坏 ,痕量的金属离子可以加速 DNA的损伤。西红花酸可以显著抑制 DNA的上述损伤。结论　西红花酸具有很强的抗自由基作用 ,对 DNA具有保护作用 Objective To study the protective effect of crocetin on DNA damage induced by different free radicals. Methods The protective effects of crocetin on DNA were detected by dextran gel, TBA reaction and UV absorption. Results After the DNA was damaged by OH · attack, the UV absorbance at 2 60 nm of the DNA decreased and the DNA deoxyribose oxidized and destroyed. The reaction between the oxidized product and TBA showed the characteristic absorption at 532 nm, the DNA chain structure was destroyed, The metal ions can accelerate DNA damage. Crocetin can significantly inhibit the above DNA damage. Conclusion crocetin has a strong anti-free radical effect on DNA has a protective effect