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本文采用高效液相色谱分离并确定了去屑止痒剂１－羟基－４－甲基－６－（２，４，４－三甲基戊基）－２－吡啶酮（以下简称ＨＭＴＰＰ）。在固定相３０１１－Ｏ柱上，以甲醇／水作流动相通过ＨＰＬＣ对保留性能进行了考察，结果表明当甲醇／水／ＨＣｌＯ＿４＝７５∶２５∶０．０５（Ｖ／Ｖ），在ＵＶ３００ｎｍ检测波长下，可实现ＨＭＴＰＰ的酸式总量测定。ＨＭＴＰＰ的线性范围为２．０～９２．０μｇ／ｍｌ，检测限为０．８０５μｇ／ｍｌ，酸式离解常数Ｋ＿ａ＝３．０５×１０－８。本方法具有分析速度快，重现性好等特点。
In this paper, high performance liquid chromatography was used to separate and identify the antidotal agent 1-hydroxy-4-methyl-6- (2,4,4-trimethylpentyl) -2-pyridone (HMTPP). Retention performance was investigated by HPLC on a stationary phase 3011-O column using methanol / water as the mobile phase. The results showed that when methanol / water / HClO 4 = 75: 25: 0.05 (V / V) Under the wavelength, the acid total amount of HMTPP can be measured. The linear range of HMTPP was 2.0 ~ 92.0μg / ml, the detection limit was 0.805μg / ml, the acid dissociation constant K_a was 3.05 × 10-8. The method has the advantages of fast analysis, good reproducibility and so on.