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通过对鲑鱼降钙素 (ｓＣＴ)和人降钙素 (ｈＣＴ)结构的比较 ,设计并合成了人降钙素类似物ｍｈＣＴ 2。利用空气氧化获得分子内二硫键 ,经分离纯化 ,产物达ＨＰＬＣ及毛细管电泳均一 ,蛋白质序列分析和质谱分析符合理论值。在大鼠降血钙生物活性测定中 ,利用量反应平行线法进行
Human calcitonin analogue mhCT 2 was designed and synthesized by comparing the structures of salmon calcitonin (sCT) and human calcitonin (hCT). The intramolecular disulfide bond was obtained by air oxidation, and the product was homogenized by HPLC and capillary electrophoresis. The protein sequence analysis and mass spectrometry were consistent with the theoretical values. In the rat blood calcium determination of biological activity, the amount of response parallel line method