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目的 :研究磷脂酶D(phospholipaseD ,PLD)是否参与胰岛素和收缩引起的I型肌纤维葡萄糖跨膜转运的信号转导体系。方法 :利用PLD的抑制剂丁醇 ,观察其对胰岛素和肌肉收缩引起的I型肌纤维占大多数的离体大鼠比目鱼肌葡萄糖跨膜转运的影响 ,了解PLD在信号转导体系中的作用。用同位素双标法测定胰岛素或肌肉收缩引起的葡萄糖跨膜转运速率 ,同时观察收缩力总量和收缩输出量时间变化曲线。结果 :同对照相比 ,丁醇可显著降低由胰岛素或肌肉收缩引起的肌细胞葡萄糖跨膜转运摄取速率 (P <0 .0 1) ,还明显影响肌肉的收缩力 (P <0 .0 1)。结论 :同在II型肌纤维中的作用一样 ,PDL也参与I型肌纤维中由胰岛素或肌肉收缩引起的肌细胞葡萄糖跨膜转运的信号转导系统。
OBJECTIVE: To investigate whether phospholipase D (PLD) is involved in the signal transduction system of glucose transport in type I muscle fiber induced by insulin and contraction. Methods: The effect of PLD on the signal transduction system was studied by using the butanol, an inhibitor of PLD. The effects of insulin-type I and muscle contraction on the transmembrane transport of glucose in isolated rat soleus muscle were observed. The isotope double labeling method was used to measure the glucose transmembrane transport rate induced by insulin or muscle contraction, and the changes of total contractile force and contractile output were observed. RESULTS: Compared with control, butanol significantly reduced glucose uptake into muscle cells (P <0.01) due to insulin or muscle contraction, and significantly affected muscle contractility (P <0.01) ). CONCLUSIONS: Like in Type II muscle fibers, PDL is also involved in the signal transduction system of muscle cell glucose transmembrane transport in type I muscle fibers induced by insulin or muscle contraction.