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目的:优选平唐复方醇提工艺和水提工艺。方法:选择提取时间、乙醇体积分数和乙醇用量为考察因素,以藁本内酯、桂皮醛、甲基丁香酚转移率及干膏得率的综合评分为指标,采用正交试验优选醇提工艺。选择浸泡时间、加水量和提取时间为考察因素,葛根素、阿魏酸转移率及干膏得率的综合评分为指标,通过正交试验优选水提工艺。结果:最佳醇提工艺为当归、桂枝和细辛药材加8,6倍量50%乙醇提取2次,每次0.5 h;藁本内酯、桂皮醛及甲基丁香酚转移率分别为72.8%,70.5%,82.3%,干膏得率27.8%。最佳水提工艺为醇提后的药渣与葛根药材浸泡30 min,加12倍量水提取3次,每次0.5 h;葛根素和阿魏酸转移率分别为60.28%和21.89%,干膏得率18.43%。结论:优选的提取工艺稳定可行,醇溶性和水溶性有效成分提取率高,为平唐复方的药效评价提供了实验依据。
Objective: To optimize Ping Tang compound alcohol extraction process and water extraction process. Methods: The extraction time, the volume fraction of ethanol and the amount of ethanol were selected as the investigation factors. Based on the comprehensive score of ligustilide, cinnamaldehyde, methyl eugenol transfer rate and the dry extract yield, the orthogonal test was used to optimize the alcohol extraction process . The soaking time, adding amount of water and extracting time were the factors of investigation. The comprehensive score of puerarin, ferulic acid transfer rate and dry extract yield was taken as index, and the optimum extraction process was orthogonal test. Results: The optimum alcohol extraction process was Angelica sinensis, Guizhi and Asarum herbs plus 8,6 times the amount of 50% ethanol extraction twice, each 0.5 h; ligustilide, cinnamaldehyde and methyl eugenol transfer rates were 72.8%, 70.5%, 82.3%, dry paste yield of 27.8%. The best water-extraction process was alcohol dregs and Radix Puerariae soaked for 30 min and 12 times of water for 3 times, each time being 0.5 h. The puerarin and ferulic acid transfer rates were 60.28% and 21.89% Cream rate of 18.43%. Conclusion: The optimal extraction process is stable and feasible, and the extraction rate of alcohol-soluble and water-soluble active ingredients is high, which provides the experimental basis for the evaluation of the efficacy of Pingtang compound.