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目的分析增加厌氧培养是否降低血小板细菌污染引起的临床输血不良反应。方法采用全自动微生物培养检测系统对9 758份单采血小板进行需氧、厌氧培养,调查单采血小板厌氧培养的细菌检出率、检出时间、检出细菌的种类以及临床输注后的反应。结果需氧培养和厌氧培养的阳性率分别为0.06%(6/9 758)和0.16%(16/9 758),增加厌氧培养使细菌污染检出率从0.06%增加到0.16%,提高了2.6倍。其中有10份血小板样本仅在厌氧培养中检出的细菌,检出的细菌以疮疱丙酸杆菌等厌氧菌为主,检出时间为(96.8±18.21)h,检出时均已临床输注,未出现不良反应。结论增加厌氧培养可提高血小板细菌污染的检出率,但是本研究中厌氧瓶未在血小板发放使用前出现阳性信号。在目前我国逐步开展血小板细菌检测的情况下,可参考北美洲和中国香港的模式首先开展需氧培养以减少血小板细菌污染引起的临床输血不良反应。
Objective To analyze whether increasing anaerobic culture can reduce clinical transfusion adverse reactions caused by platelet bacterial contamination. Methods Nine 758 apheresis plates were cultured aerobically and anaerobically with an automatic microbe culture system. The detection rate, detection time, bacteria types and clinical infants after anaerobic culture of apheresis platelets were investigated. Reaction. Results The positive rates of aerobic culture and anaerobic culture were 0.06% (6/9 758) and 0.16% (16/9 758) respectively. Increasing anaerobic culture increased the detection rate of bacterial contamination from 0.06% to 0.16% 2.6 times. Among them, 10 of the platelet samples were detected only in anaerobic culture. The detected bacteria were mainly anaerobic bacteria such as Propionibacterium acnes. The detection time was (96.8 ± 18.21) h, both of which were detected Clinical infusion, no adverse reactions. Conclusion Increasing anaerobic culture can increase the detection rate of platelet bacterial contamination, but the anaerobic bottle in this study did not show a positive signal before platelet release. In the current situation of gradual detection of platelet bacteria in our country, we can refer to the patterns of North America and Hong Kong, China for the first time to carry out aerobic culture to reduce the clinical transfusion adverse reactions caused by platelet bacterial contamination.