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用神经氨酸酶及羧肽酶B处理血浆,依次经C4高压琼脂糖电泳、免疫固定、薄层激光扫描等步骤,并结合血浆免疫火箭电泳定量法对我国武汉地区随机人群的血浆C4总量及C4两种同种型,主要别型,单、双QO,重复座位等时的血浆C4含量进行了检测与分析。结果发现:遗传因素明显影响血浆C4水平,因而在群体中表现宽广的C4浓度范围,特别在有C4QO与C4重复基因座位时差异特别明显。这一研究提示,不同的C4基因型,循环C4含量表现明显差异。临床上检测C4含量时应该考虑患者的C4基因型别。
The plasma was treated with neuraminidase and carboxypeptidase B, followed by C4 high-pressure agarose electrophoresis, immunofixation, thin-layer laser scanning and other steps, combined with the plasma immune rocket electrophoresis quantitative method to determine the total amount of plasma C4 And C4 two isoforms, the main type, single, double QO, repeated seat plasma C4 concentrations were detected and analyzed. The results showed that: genetic factors significantly affect the level of plasma C4, and thus in the population showed a wide range of concentrations of C4, especially in C4QO and C4 repeat loci particularly significant differences. This study suggests that different C4 genotypes, circulating C4 content showed significant differences. Clinic C4 content should be considered when the patient’s C4 genotype.