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目的探讨金葡菌L型感染在卵巢癌中检测的意义。方法应用革兰染色、免疫组织化学染色技术检测HO-8910PM细胞株以及97例卵巢乳头状癌、23例卵巢乳头状瘤石蜡包埋组织中细菌L型的感染情况。结果(1)HO-8910PM细胞与金葡菌L型体外共培养后在肿瘤细胞的胞浆及胞核中检测到L型的阳性表达。(2)卵巢乳头状癌和乳头状瘤组织中,细菌L型感染率分别为25.8%(25/97)和13.0%(3/23)。L型检出率与卵巢癌的临床分期、病理分级及腹腔淋巴结转移的差异均有显著性(P<0.005),与组织学类型无关(P>0.05)。结论金葡菌L型可以进入细胞内,并能够在一定程度上促进卵巢癌的发生、发展。
Objective To investigate the significance of Staphylococcus aureus L-type infection in ovarian cancer. Methods Gram stain and immunohistochemical staining were used to detect the bacterial L-type infection in HO-8910PM cell line and in 97 cases of ovarian papillary carcinoma and 23 cases of ovarian papilloma paraffin-embedded tissues. Results (1) The positive expression of L-type was detected in the cytoplasm and nucleus of HO-8910PM cells co-cultured with L-form S. aureus. (2) The infection rate of bacterial L-forms was 25.8% (25/97) and 13.0% (3/23) in papillary and papilloma tissues respectively. There was a significant difference between the detection rate of L type and the clinical stage, pathological grade and lymph node metastasis of ovarian cancer (P <0.005), but not with histological type (P> 0.05). Conclusion Staphylococcus aureus L-type can enter the cell and can promote the occurrence and development of ovarian cancer to a certain extent.