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海风藤酚、甲基海风藤酚、海风藤醇Ａ和海风藤醇Ｂ均有抑制血小板激活因子（ＰＡＦ）诱导的兔血小板聚集作用，ＩＣ_（５０）分别为１７．９、９．２、１４２．０、１４．０μｍｏｌ·Ｌ~（－１）。经阿司匹林（ＡＳＡ）、磷酸肌酸／磷酸肌酸激酶（ＣＰ／ＣＰＫ）预处理的兔洗涤血小板，甲基海风藤酚和海风藤醇Ｂ仍能抑制ＰＡＦ诱导的兔血小板聚集，ＩＣ_（５０）为９．０、１６．５μｍｏｌ·Ｌ~（－１）。同时，４种成分对于二磷酸腺苷（ＡＤＰ），花生四烯酸（ＡＡ）诱导的血小板聚集抑制作用较弱，海风藤Ｂ能使ＰＡＦ诱导血小板聚集量－效曲线平行右移，ＥＣ_（５０）由１．５９ｎｍｏｌ·Ｌ~（－１）升为２０．６ｎｍｏｌ·Ｌ~（－１）。结果表明：４种成分能选择性拮抗ＰＡＦ对兔血小板的聚集作用。
Nematocin, Methylprednisolol, Trifluralin A and Trifluralin B all inhibited platelet aggregation induced by platelet activating factor (PAF), the IC 50 values were 17.9, 9.22 and 142 .0,14.0μmol·L -1. Platelet pretreatment with aspirin (ASA), creatine phosphate / creatine phosphokinase (CP / CPK), and platelet-carbinol (MTT) 9.0, 9.5μmol·L -1. At the same time, the inhibition of 4 components on ADP and AA induced platelet aggregation was weak, and the inhibition of PAF-induced platelet aggregation- ) Increased from 1.59nmol·L -1 to 20.6nmol·L -1. The results showed that the four components could selectively antagonize the aggregation of PAF on rabbit platelets.