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目的观察R,R-福莫特罗(R,R-FMT)对慢性阻塞性肺疾病(COPD)大鼠气道高反应性、气道炎症以及肺组织形态学的影响。方法经反复气道内注入木瓜蛋白酶和脂多糖建立大鼠COPD模型,通过大鼠基础气道阻力(RL)和不同浓度乙酰甲胆碱(Mch)诱导的RL的变化、支气管肺泡灌洗液(BALF)中炎症细胞以及肺组织形态学的改变,评价R,R-FMT的作用。结果COPD模型组大鼠的RL为(0.23±0.02)cmH2O·ml-1·s-1,RL增加50%的Mch浓度(PC50)为(4.31±1.33)g/L;而对照组的RL为(0.15±0.02)cmH2O·ml-1·s-1,PC50为(9.84±3.95)g/L;R,R-FMT用药组的RL为(0.17±0.01)cmH2O·ml-1·s-1,PC50为(7.34±3.21)g/L,3组比较差别有显著性意义(F=29.71、4.99,P<0.01、<0.05)。模型组BALF中的炎症细胞总数为(9.83±0.80)×108/L、中性粒细胞为(1.88±0.46)×108/L、淋巴细胞为(3.77±0.71)×108/L、单核/巨噬细胞为(4.19±0.76)×108/L;而对照组分别为(0.95±0.25)×108/L、(0.05±0.03)×108/L、(0.12±0.06)×108/L、(0.77±0.16)×108/L;R,R-FMT用药组则分别为(3.82±0.44)×108/L、(0.35±0.12)×108/L、(0.74±0.24)×108/L、(2.73±0.31)×108/L;3组比较差别亦有显著性意义(F=412.79、75.56、121.58、75.80,均P<0.01)。此外,用R,R-FMT干预可使COP
Objective To observe the effects of R, R-FMT on airway hyperresponsiveness, airway inflammation and lung histomorphology in chronic obstructive pulmonary disease (COPD) rats. Methods COPD model was established by repeated instillation of papain and lipopolysaccharide in rats. The bronchoalveolar lavage fluid (BALF) was induced by the changes of basal airway resistance (RL) in rats and RL induced by different concentrations of methacholine (Mch) ) In inflammatory cells and lung tissue morphology changes, evaluate the role of R, R-FMT. Results The RL of the COPD model group was (0.23 ± 0.02) cmH2O · ml-1 · s-1 and the PC50 value of the RL group increased by 50% (4.31 ± 1.33) g / L, while the RL of the control group (0.15 ± 0.02) cmH2O · ml-1 · s-1, and PC50 was (9.84 ± 3.95) g / L. The RL of R, R-FMT group was (0.17 ± 0.01) cmH2O · ml-1 · s-1 , PC50 (7.34 ± 3.21) g / L, the difference between the three groups was significant (F = 29.71, 4.99, P <0.01, <0.05). The total number of inflammatory cells in BALF in model group was (9.83 ± 0.80) × 108 / L, neutrophil (1.88 ± 0.46) × 108 / L and lymphocyte (3.77 ± 0.71) × 108 / (4.19 ± 0.76) × 108 / L and (0.95 ± 0.25) × 108 / L and (0.05 ± 0.03) × 108 / L and (0.12 ± 0.06) × 108 / L and 0.77 ± 0.16) × 108 / L respectively; (3.82 ± 0.44) × 108 / L, (0.35 ± 0.12) × 108 / L and (0.74 ± 0.24) × 108 / L for R and R- 2.73 ± 0.31) × 108 / L. The difference between the three groups was also significant (F = 412.79, 75.56, 121.58, and 75.80, all P <0.01). In addition, R, R-FMT intervention can COP