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目的：研究突变型ｐ５３和ｃ－ｎｅｕ癌基因产物在胃癌中的表达。方法：用免疫组化方法检测４７例胃癌组织中突变型ｐ５３蛋白和ｃ－ｎｅｕ癌基因产物的表达。结果：４７例胃癌组织中ｐ５３的阳性率为６１．７％，可分为胞浆型、核型以及全细胞型。胞浆型与胃癌高分化有关，核型与胃癌低分化有关（Ｐ＜０．０１）。２例早期胃癌中，１例呈胞浆ｐ５３阳性，另１例呈ｐ５３阴性。ｃ－ｎｅｕ阳性率为９１．５％，与胃癌组织学分型无关，多分布在胃癌细胞的胞浆及胞膜，少数在核膜上。突变型ｐ５３和ｃ－ｎｅｕ共同表达率为４８．９％。结论：突变型ｐ５３和ｃ－ｎｅｕ癌基因在胃癌发生、发展过程中具有协同作用。
Objective: To study the expression of mutant p53 and c-neu oncogene products in gastric cancer. Methods: Immunohistochemistry was used to detect the expression of mutant p53 protein and c-neu oncogene product in 47 cases of gastric cancer. RESULTS: The positive rate of p53 in 47 cases of gastric cancer was 61.7%, which could be divided into cytoplasm, karyotype and whole cell type. The cytoplasmic type was related to the high differentiation of gastric cancer, and the karyotype was related to the poor differentiation of gastric cancer (P<0.01). In 2 cases of early gastric cancer, 1 case was cytoplasmic p53 positive and the other 1 was p53 negative. The positive rate of c-neu was 91.5%, which was not related to the histological type of gastric cancer. It was mostly distributed in the cytoplasm and membrane of gastric cancer cells, and a few in the nuclear membrane. The co-expression rate of mutant p53 and c-neu was 48.9%. Conclusion: The mutant p53 and c-neu oncogenes have a synergistic effect in the development and progression of gastric cancer.